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Furthermore, heterologous expression of MeUGT1 in Arabidopsis thaliana increased the flavonol glycoside contents in the plants. Therefore, the UGTs characterized in this study could provide new data that will be useful for examining flavonoid biosynthesis in liverworts.Melatonin (N-acetyl-5-methoxytryptamine) plays important roles in the regulation of development and the response to biotic and abiotic stresses in plants. Serotonin-N-acetyltransferase (SNAT) functions as a key catalytic enzyme involved in melatonin biosynthesis. In this study, the candidate gene VvSNAT1 (SNAT isogene) was isolated from grape (Vitis vinifera L. cv. Merlot). Tissue-specific expression and external treatment revealed that VvSNAT1 is a salt-inducible gene that is highly expressed in leaves. Subcellular localisation results revealed that VvSNAT1 was located in the chloroplasts, which is similar to other plant SNAT proteins. Ectopic overexpression of VvSNAT1 in Arabidopsis resulted in increased melatonin production and salt tolerance. Transgenic Arabidopsis overexpressing VvSNAT1 exhibited enhanced growth and physiological performance, including a lower degree of leaf wilting, higher germination rate, higher fresh weight, and longer root length under salt stress. Isoarnebin 4 Moreover, overexpression of VvSNAT1 in Arabidopsis protected cells from oxidative damage by reducing the accumulation of malondialdehyde (MDA) and hydrogen peroxide (H2O2). These results indicate that VvSNAT1 positively responds to salt stress. Our results provide a novel perspective for VvSNAT1 to improve salt tolerance, mediated by melatonin accumulation, plant growth promotion and oxidative damage reduction.Sinocalycanthus chinensis, a diploid (2n = 22) deciduous shrub, belongs to the Calycanthaceae family of magnoliids and is rich secondary metabolites, such as terpenoids. However, the regulation of terpenoid biosynthesis in S. chinensis is largely unknown. In this study, comparative transcriptome analyses were performed in the bark, branches, leaves, and flowers. KEGG enrichment analysis revealed that the terpenoid biosynthesis and cytochrome P450 pathways were significantly enriched in the four tissues. Twelve terpenoid backbone biosynthesis-related genes were identified, and eight terpene synthases (TPSs) were reassembled based on independent transcriptomes from the four tissues. Phylogenetic analysis of the TPSs showed high sequence similarity between S. chinensis and Arabidopsis, and these TPSs were classified into three subfamilies. Moreover, 39 phytohormone response-related genes, including 5 abscisic acid (ABA) receptors, 25 auxin response factors, 3 gibberellin (GA) response genes, 5 ethylene response genes, and 1 jasmonic acid (JA) response gene were analyzed. Most phytohormone pathway-related genes were upregulated in the flowers and downregulated in the leaves. The endogenous indole acetic acid (IAA) content was higher in the flowers than in the other comparisons. Our results provide an opportunity to reveal the regulation of terpenoid biosynthesis in S. chinensis.Aluminum oxide and zinc oxide nanoparticles (NPs) are two of the mostly produced engineered metal oxide NPs. Here, barley germination and root elongation as well as gene expressions of the selected aquaporins (HvTip1;1 and HvPip1;1) and transcription factors (HvERFs and HvNFX1) were investigated after exposure to Al2O3 and ZnO NPs for foreseeing the effect of NP exposure. ICP-MS analysis showed that the nanoparticles were taken up into root and leaves. Even the germination analysis and seedling establishment data indicate that the applied NPs do not have any observable inhibitory effects except on root length, the gene expression analysis revealed that these nanoparticle applications lead to a response at the molecular level. The gene expression profiling indicated that aquaporins and transcription factor genes were differentially regulated in leaves and roots in response to NPs treatments. The expressions of aquaporin genes were higher especially in leaves in compared to the control plants. Gradual decrease was obtained in roots by application of the increased levels of Al2O3 NPs. The effects of ZnO NPs on gene expression levels of barley TFs were dramatically more distinctive in comparison with that of Al2O3 NPs. The expression profiles of HvERFs and HvNFX1 transcription factors in response to the Al2O3 and ZnO NPs suggest that these selected TFs can play important roles in shaping abiotic stress tolerance in young barley roots and leaves. Outcomes of the study will allow us to predict complex stress response of barley in response to the nanoparticles.Terrestrial carnivorous plants of genera Drosera, Dionaea and Nepenthes within the order Caryophyllales employ jasmonates for the induction of digestive processes in their traps. Here, we focused on two aquatic carnivorous plant genera with different trapping mechanism from distinct families and orders Aldrovanda (Droseraceae, Caryophyllales) with snap-traps and Utricularia (Lentibulariaceae, Lamiales) with suction traps. Using phytohormone analyses and simple biotest, we asked whether the jasmonates are involved in the activation of carnivorous response similar to that known in traps of terrestrial genera of Droseraceae (Drosera, Dionaea). The results showed that Utricularia, in contrast with Aldrovanda, does not use jasmonates for activation of carnivorous response and is the second genus in Lamiales, which has not co-opted jasmonate signalling for botanical carnivory. On the other hand, the nLC-MS/MS analyses revealed that both genera secreted digestive fluid containing cysteine protease homologous to dionain although the mode of its regulation may differ. Whereas in Utricularia the cysteine protease is present constitutively in digestive fluid, it is induced by prey and exogenous application of jasmonic acid in Aldrovanda.Lysophosphatidic acid (LPA) signaling plays diverse roles in the development of various vertebrates such as mammals and fish. The lamprey is a fish that retains ancestral features of vertebrates, but information regarding lamprey LPA receptor genes is limited. Here, using information from the lamprey genome database, we cloned two LPA receptor genes, Lpar1 and Lpar5, from the Japanese lamprey (Lethenteron camtschaticum). Lamprey Lpar1 had a high amino acid identity to mouse and medaka fish Lpar1, whereas Lpar5 amino acid sequences were more diverse between species. Our functional analyses using a heterologous expression system demonstrated that Lpar1 and Lpar5 responded to LPA treatment with G12/13-associated cellular responses, which are indicative of cytoskeletal actions. The existence of functional LPA receptors in the Japanese lamprey suggests that LPA receptor-dependent signals contribute to lamprey growth and development.