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OBJECTIVES Characterisation of tumor-infiltrating lymphocytes (TILS) population for cancer prognostication has enabled deeper understanding of tumor immune interactions in cancer immunology. We aim to examine the significance of both the density and functional status of NK cells in a cohort of Epstein Barr Virus (EBV) associated Nasopharyngeal Cancer (NPC) patients. METHODS NK TILS of 50 NPC samples were quantified on immunohistochemistry and the density of NK TILS was correlated with clinical outcomes. Next, NK cells and a panel of cytokines of 10 newly diagnosed NPC patients were characterized in both NPC tissue and peripheral circulation. Exhausted NK cells were identified using co-expression of PD-1 and/or Tim-3. Comparison of percentage of NK cells in NPC and healthy controls was performed using student t-test for two groups; and a p value of less than 0.05 values was considered significant. RESULTS NK TILS exhibited a bimodal distribution; with the NKhigh cohort demonstrating a poorer 2-year overall survival rate (p  less then  0.035). In-vitro studies revealed a higher proportion of infiltrated NK cells in the NKhigh cohort co-expressed PD-1. Additionally, IL-18 levels in NPC tissue were significantly higher than in healthy nasopharynx; and IL-18 alone induced PD-1 expression on NK cells. Expectedly, plasma IL-18 concentration and percentage of circulating PD-1-expressing NK cells were similar among NPC patients and healthy controls. CONCLUSION The cytotoxic function of NK TILS is mitigated by an elevated IL-18 levels within the NPC microenvironment. Hence, the functional status, and the density of NK cells in TILS should be considered when prognosticating NPC. There are a number of studies that have conducted comparisons of body appreciation between males and females. However, findings are largely inconsistent, making it unclear whether there are actual gender differences in body appreciation. With a meta-analytic approach, the current study quantitatively reviewed and synthesized previous findings, published up to May 2019, on gender differences in body appreciation. After searching and screening potential studies in four databases (i.e., PubMed, PsycINFO, Web of Science, and ProQuest Dissertations & Theses Global), we identified 40 relevant articles published from 2008 to 2019. A random-effects model reveals an overall estimate of gender difference in body appreciation of d = 0.27 (95 % CI 0.21, 0.33; p  less then   .001); that is, males generally have a higher level of body appreciation than females, with a small effect size. Survey method, type of sample (cohorts), and age were identified as significant moderators that have contributed to the variability in previous findings. Future research and interventions in body appreciation may consider gender differences in their designs. One increasing trend on social media is the posting of body positive content that aims to challenge narrow beauty ideals and instead promote acceptance and appreciation of all bodies. The aim of the present study was to experimentally investigate the effect of body positive captions attached to Instagram images on young women’s body image. Participants were 384 women aged 18-30 years randomly assigned to view Instagram images of thin or average-sized women containing either body positive captions or no captions. In contrast to prediction, the body positive captions had no effect on body dissatisfaction or body appreciation. There was a significant effect of image type, whereby the average images resulted in less body dissatisfaction and greater body appreciation than the thin images. A significant three-way interaction indicated that for women high on thin-ideal internalisation, body positive captions on average images led to greater body appreciation, but lower body appreciation when attached to thin images. The results suggest that the visual imagery of an Instagram post is a more potent contributor to body image than any accompanying text. Presenting a more diverse array of women’s bodies on social media is likely a more effective way to foster body satisfaction and appreciation. Epithelioid hemangioendothelioma (EHE) is a rare malignant angiocentric vascular neoplasm. Around 90% of classic EHE has a t(1;3)(p36;q25) that results in a WWTR1-CAMTA1 fusion gene, a histologically distinctive subset of EHE has been recently shown to have a t(10;14)(p13;q42)that results in a different fusion gene, YAP1-TFE3. Twenty-one cases of TFE3 Rearranged Epithelioid Hemangioendothelioma have been reported in the literature, and only two cases occurred in bone. In the report, we report additional two cases occurred in the femur and skull and review the related literature. Indirect (IBil), direct (DBil) and total (TBil) bilirubin are important clinical indicators of hepatobiliary diseases, which require rapid detection in diagnosis and treatment. IBil and DBil have a structural relationship with several macromolecules in hepatobiliary metabolism. Here, the rapid analysis models for bilirubin indicators using serum visible-near-infrared (Vis-NIR) spectroscopy were established. Norris derivative filter with optimisation was used for spectral pretreatment; the optimal parameters (derivative order, number of smoothing points, number of differential gaps) were (2, 15, 9) for IBil; (2, 13, 9) for DBil, respectively. selleck compound Equidistant combination-partial least squares (EC-PLS) was used for large-scale wavelength screening. Wavelength step-by-step phase-out PLS (WSP-PLS) was used for secondary wavelength optimisation. The wavelength models of the optimal EC-WSP-PLS for IBil and DBil included 11 and 18 wavelengths, respectively. In independent validation, the root-mean-square errors and correlation coefficient for prediction (SEP, RP), and ratio of performance-to-deviation (RPD) were 0.90 μmol L-1, 0.975, and 4.4 for IBil; 0.71 μmol L-1, 0.955, and 3.3 for DBil, respectively. TBil was subjected to spectral analysis, and the summation of the prediction values of IBil and DBil was compared. The latter was obviously better, and SEP, RP, RPD were 0.82 μmol L-1, 0.990, 7.1, respectively. The results for IBil, DBil and TBil indicated high correlation, low error and good overall prediction ability and confirmed the feasibility of the simultaneous analysis of bilirubin indicators through reagent-free serum Vis-NIR spectroscopy. The proposed method is crucial for the rapid screening of large populations and the treatment of hepatobiliary diseases. V.2-(2′-Hydroxyphenyl) thiazole-4-carboxaldeyde (aldehyde 1) and hemiacetal 2 were selected to study the mechanism of excited-state intramolecular proton transfer and the detecting of Al3+ ion in methanol by using density functional theory (DFT) and time-dependent density functional theory (TDDFT) methods. Our theoretical results are in good agreement with the experimental values. The intramolecular H-bond is enhanced in the first excited-state based on the analyses of structural parameters, frontier molecular orbitals and electronic spectra. The stronger intramolecular H-bond is more favorable for ESIPT process. In order to further demonstrate the proton transfer process, we constructed the potential energy curves of probe 1 and 2 in both ground- and excited-states, and concluded that proton transfer processes in probe 1 and 2 are apt to happen in the S1 state. In addition, the Mayer bond order, energy gap and absorption and fluorescence spectra were applied to interpret the process of detection of Al3+ ion. The capability of citrate-stabilized gold nanoparticles (AuNps) has been explored for the inhibition of amyloid fibrillation of human γD-crystallin (HGD), a major protein of eye lens. Citrate-capped AuNps were synthesized, characterized and used further for amyloid inhibition. The results from intrinsic and extrinsic (in the presence of Thioflavin T and ANS) fluorescence based assays and CD spectroscopy clearly suggest that AuNps at nanomolar concentrations can act as an effective inhibitor against fibrillation of HGD. Fluorescence microscopic and transmission electron microscopic images also supported this observation. Considering the inhibitory role of AuNps against HGD fibrillation, interactions between HGD and AuNps were studied to decipher the mechanism of amyloid inhibition. The binding and quenching constants were calculated as ~109 M-1 using the data of tryptophan fluorescence quenching of HGD by AuNps. Ground state complexation between the protein and nanoparticles was predicted. AuNps were not found to cause any major conformational changes in the native protein. Entropy-driven complexation process between the protein and nanoparticles indicates the interactions of AuNps with hydrophobic residues of HGD. Therefore, in the presence of AuNps, the exposure of the hydrophobic patches of HGD during its partial unfolding became restricted, which results inhibition in HGD fibrillation. Indolium derivatives bearing diphenyl amine (R1) and dibenzylamine (R2) linked through benzene was designed, synthesized, characterized and its cyanide recognizing abilities are studied in 100% water. The probes were exhibited dual channel properties i.e. both colorimetric and fluorimetric changes for the effective sensing of cyanide ion. The discrete color variation of probes with cyanide ion makes possible as colorimetric sensor and which was seen easily through naked eye. The recognition of cyanide ion is accomplished via the attachment of cyanide ion at the C atom of indolium CN group through nucleophilic addition. Also, the found detection limits of probes (nM range) were much lower than the WHO limit. Besides, the probes were effectively applied to determine cyanide ion in real water samples and thus was confirmed with HPLC method. Herein we describe a simple fluorescence quenching method for the selective recognition and determination of the amino acid phenylalanine (Phe). The use of 1H NMR spectroscopy revealed that the alkaloid palmatine (PAL) can encapsulated partially into the cavity of cucurbit[7]uril (Q[7]) in aqueous solution to form a stable 11 host-guest inclusion complex. This host-guest complex exhibits fluorescence of moderate intensity. Interestingly, the addition of the Phe results in a dramatic quenching of the fluorescence intensity associated with the inclusion complex. By contrast, the addition of other natural amino acids resulted in no change in the fluorescence. Based on the linear relationship between the fluorescence intensity and the concentration of Phe, the detection of the concentration of Phe in aqueous solution is facile. Thus, a new fluorescence quenching method for the recognition and determination of the Phe has established herein. Single crystals of α-quartz-type Si1-xGexO2 (x less then  0.12), grown under hydrothermal conditions in NH4F solutions, were investigated using infrared (IR) and Raman spectroscopy. Compositional dependencies of the IR absorption spectra were found both for the fundamental and combination vibrations. With an increase in Ge content in the crystals, new absorption bands, corresponding to the vibrations of OGeO in the GeO4 tetrahedra (670, 930, 1010, 2125 cm-1), appeared, and the intensities of the absorption bands of OSiO in the SiO4 tetrahedra (263, 695, 2137, doublet at 2326 and 2333, 2499, 2599, 2673 cm-1) decreased. The shifts in the absorption bands at 354, 511, 2499 and 2673 cm-1 to lower wavenumbers linearly depends on the Ge content and are characteristic of vibrations in the SiOGe chains. The origin of some combinational vibrations is also clarified. A combination vibration that caused the band at 1395 cm-1 was formed by vibrations with wavenumbers of 263 (type E) and 1170 cm-1 (type E). The bands at 2499 and 2673 cm-1 were composed of the band at 354 cm-1 and two vibrations at 1083 cm-1 (type E) and by two vibrations at 1170 cm-1 (type E), respectively.