Activity

Knee kinematics in the simulation showed similar trends to the clinical in vivo data and were almost within the range of inter-specimen variability.

Postoperative knee kinematics in BCS TKA differed according to PTS and anterior post-cam contact; in particular, anterior post-cam contact changed knee kinematics, which may affect the patient’s perception of the knee during activities. Cite this article

2020;9(11)761-767.

Postoperative knee kinematics in BCS TKA differed according to PTS and anterior post-cam contact; in particular, anterior post-cam contact changed knee kinematics, which may affect the patient’s perception of the knee during activities. Cite this article Bone Joint Res 2020;9(11)761-767.Early goal-oriented conversations at the end of life can help alleviate suffering and anxiety for surgical patients with cancer and their loved ones. This is especially important in the young adult population in whom there are limited patient-reported perspectives about their experiences at the end of life. We report the illustrative case of a 29-year-old woman who underwent palliative surgery for widely metastatic renal cell carcinoma. Her postoperative course was complicated by a proximal anastomotic stricture, bilateral pulmonary emboli, and delayed gastric emptying, ultimately leading to a functional decline in health and quality of life. Several wide-ranging palliative care needs arose during our patient’s surgery, discharge, and subsequent readmissions. In addition to our patient’s case, we discuss 3 fundamental surgical palliative care principles, and broad considerations for end-of-life management of young adults with advanced malignancies.

This study aimed to investigate the effect of

(

) gene mutation (identified from a hereditary multiple exostoses family) on chondrocyte proliferation and differentiation.

ATDC5 chondrocytes were cultured in insulin-transferrin-selenium medium to induce differentiation. Cells were transfected with pcDNA3.0 plasmids with either a wild-type (WT) or mutated (MUT)

gene. The inorganic phosphate (Pi) concentration in the medium of cells was determined. The expression of markers of chondrocyte proliferation and differentiation, the Indian hedgehog (Ihh), and parathyroid hormone-related protein (PTHrP) pathway were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting.

The expression of

in MUT group was similar to WT group. The Pi concentration in the medium of cells in MUT group was significantly higher than WT group, which meant the

mutation inhibited Pi uptake in ATDC5 chondrocytes. The proliferation rate of ATDC5 chondrocytes in MUT group was greater than Wutation promotes chondrocyte proliferation while inhibiting chondrocyte differentiation. The Ihh/PTHrP signalling pathway may play an important role in this process. Cite this article Bone Joint Res 2020;9(11)751-760.Fiber strain sensors that are directly woven into smart textiles play an important role in wearable systems. These sensors require a high sensitivity to detect the subtle strain in practical applications. However, traditional fiber strain sensors with constant diameters undergo homogeneous strain distribution in the axial direction, thereby limiting the sensitivity improvement. Bismuth subnitrate chemical Herein, a novel strategy of internal or external structural design is proposed to significantly improve the sensitivity of fiber strain sensors. The fibers are produced with directional increases in diameter (internal design) or polydimethylsiloxane (PDMS) microbeads attached to surfaces (external design) by combining hollow glass tubes used as templates with PDMS drops. The structural modification of the fiber significantly impacts the sensing performance. After optimizing structural parameters, the highest gauge factor reaches 123.1 in the internal-external structure design at 25% strain. A comprehensive analysis reveals that the desirable scheme is the internal structural design, which features a high sensitivity of 110 with a 100% improvement at ∼5-20% strain. Because of the sufficiently robust interface, even at the 800th cycle, fiber sensors still possessed an excellent stable performance. The morphology evolution mechanism indicates that the resistance increase is closely related with the increased peak width and distance, and the appearance of gaps. Based on the finite element modeling simulation, the quantified effective contributions of different strategies positively correlate with the improved sensitivity. The proposed fiber strain sensors, which are woven into the two-dimensional network structure, exhibit an excellent capability for displacement monitoring and facilitate the traffic control of crossroads.Fine particulate matter (PM2.5)-induced metabolic diseases have attracted a great deal of attention recently. However, the relevant metabolic mechanisms of PM2.5 in vivo have not yet been fully described due to the lack of reliable platforms. Herein, a membrane-free liver-gut-on-chip (L-GOC) platform was developed to investigate metabolism dysregulation induced by PM2.5. A multiple organ system with a liver-gut structure and two circulation paths (L-G and G-L circulation paths) was created, and then cells were exposed to PM2.5 on this platform. Secreted high-density lipoprotein (HDL) levels were detected, which demonstrates that this multiple organ system functioned with normal physiological metabolism at the organ level. Untargeted metabolomic analysis showed that there were 364 metabolites of LO2 cells dysregulated after exposure to PM2.5 at a concentration of 200 μg/mL. Moreover, cholesterol and bile acid metabolism were significantly dysregulated. Further immunofluorescence and ELISA assays confirmed that signal transduction pathways related to cholesterol metabolism (LCAT-CE, PON1-HDL, and SRB1-HDL metabolic pathways) and bile acid metabolism (CYP7A1-CA/CDCA/DCA metabolic pathways) were disturbed. These results indicate that PM2.5 primarily disturbed cholesterol metabolism of the liver and then disrupted bile acid metabolism of the liver (primary bile acid biosynthesis) and gut (secondary bile acid biosynthesis) via related metabolic pathways. These findings may partially explain the metabolic mechanisms of cells triggered by PM2.5 exposure.