Activity

oprotective potential in terms of reducing urea and creatinine concentrations.

The findings suggest that CEFA possesses antilipidaemic properties in terms of elevation and decrease in HDL and TAG concentrations respectively. Also, CEFA possesses renoprotective potential in terms of reducing urea and creatinine concentrations.

Gamma-aminobutyric acid (GABA), with an antidepressant effect, and Monacolin K, with a cholesterol-lowering effect, are the main bioactive ingredients in Monascus-fermented rice (MFR). The simultaneous enrichment of both ingredients can effectively enhance the health benefits of MFR. However, the capacity of Monascus spp. to produce GABA is limited.

Seventeen lactic acid bacteria (LAB) strains were preliminarily screened for GABA-producing by whole-cells bioconversion of L-glutamate, followed by rescreening through fermentation with the addition of the precursor L-glutamic acid. Subsequently, the bioconversion conditions (temperature, metal ions, and pH) for the conversion of L-monosodium glutamate (MSG) were investigated. Additionally, the GABA-producing LAB was co-inoculated with a monacolin K producing strain Monascus anka 20-2, and the ratio of M. anka 20-2 to LAB in microbial consortia was optimized for MFR production.

The strain Lactobacillus plantarum 8014 was screened out for its ability to prodid bacteria.

ch aims to assess the effect of Calotropis procera plant stems as a coagulant treatment on the Wangashi cheese in order to characterize and standardize its production. Crude extract obtained from the Calotropis plant stems added to milk powder were used as a solution dissolved at various pH (4-8) and temperature (35-80°C) in order to examine the effect of pH and temperature on milk clotting and proteolytic activities. The pH 5.5 and temperature of 70°C were recorded as optimum pH and temperature. After that the concentration of the crude extract enzyme was assayed to purify it using ammonium sulfate precipitation at various percentage of saturation (20-80%) at determined optimum pH and temperature, whereby the saturation of 70% was detected to be the best because of its high specific activity, yield and purification fold. Two types of Wangashi cheese were produced in laboratory, one using directly the crude extract and the other the purified crude extract from Calotropis procera at optimum condition. Their the purified crude extract from Calotropis procera at optimum condition. Their chemical, textural and color properties were determined using standard methods. A significant difference between parameters tested was observed (p less then 0.05). A decrease in moisture content, increase in protein content and also an improvement of color and textural parameters were recorded for the cheese obtained using purified crude extract Calotropis procera staems.

The majority of the neurologically impaired persons, who are suffering from Spinal Cord Injury (SCI) or Multiple Sclerosis (MS), may face important lifestyle restrictions. Urinary disorders can further decrease their Quality of Life (QoL). SF Qualiveen is a validated questionnaire for the evaluation of QoL in this population related to Neurogenic Lower Urinary Tract Dysfunction (NLUTD).

The cross-cultural adaptation, the reliability check, and the validation of the SF Qualiveen in Greek.

Observational cohort study.

Between November 2019 and May 2020, we addressed to 136 consecutive neurologic patients with MS or SCI from the outpatient clinic of the Neuro-urology Unit of the National Rehabilitation Centre in Athens.

124 patients (68 males and 56 females) completed the study. There were 55 paraplegics, 16 tetraplegics, and 53 MS patients.

After a back forward translation of the SF Qualiveen between English and Greek, the patients completed the Greek version of SF Qualiveen and King’s Health Questioek population.

This Greek version of the SF Qualiveen can be used as a tool to evaluate the impact of NLUTD on QoL in Greek-speaking patients with MS and SCI in research and clinical practice.

This Greek version of the SF Qualiveen can be used as a tool to evaluate the impact of NLUTD on QoL in Greek-speaking patients with MS and SCI in research and clinical practice.Not available.Not available.Not available.Histological transformation into diffuse large B-cell lymphoma is a rare complication in patients with Waldenström macroglobulinemia (WM) usually associated with a poor prognosis. The objective of this study was to develop and validate a prognostic index for survival in transformed WM patients. Through this multicenter, international collaborative effort, we developed a scoring system based on data from 133 patients with transformed WM who were evaluated between 1995 and 2016 (training cohort). Univariate and multivariate analyses were used to propose a prognostic index with 2-year survival after transformation as an end-point. read more For external validation, a data set of 67 patients was used to evaluate the performance of the model (validation cohort). By multivariate analysis, three adverse covariates were identified as independent predictors of 2-year survival after transformation elevated serum LDH (2 points), platelet count less then 100 x 109/L (1 point) and any previous treatment for WM (1 point). Three risk groups were defined low-risk (0-1 point, 24% of patients), intermediate-risk (2-3 points, 59%, hazard ratio (HR) = 3.4) and high-risk (4 points, 17%, HR = 7.5). Two-year survival rates were 81%, 47%, and 21%, respectively (P less then 0.0001). This model appeared to be a better discriminant than the International Prognostic Index (IPI) and the revised IPI (R-IPI). We validated this model in an independent cohort. This easy-to-compute scoring index is a robust tool that may allow identification of groups of transformed WM patients with different outcomes and could be used for improving the development of risk-adapted treatment strategies.Next-Generation Sequencing has recently been introduced to efficiently and simultaneously detect genetic variations in acute myeloid leukemia. However, its implementation in the clinical routine raises new challenges focused on the diversity of assays and variant reporting criteria. To overcome this challenge, the PETHEMA group established a nationwide network of reference laboratories aimed to deliver molecular results in the clinics. We report the technical cross-validation results for next-generation sequencing panel genes during the standardization process and the clinical validation in 823 samples of 751 patients with newly diagnosed or refractory/relapse acute myeloid leukemia. Two cross-validation rounds were performed in seven nationwide reference laboratories in order to reach a consensus regarding quality metrics criteria and variant reporting. In the pre-standardization cross-validation round, an overall concordance of 60.98% was obtained with a great variability in selected genes and conditions across laboratories.