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LncRNA HULC silencing had opposite effects on the tubular formation, invasion, and EMT process as well as tumor growth of GBM cells.

LncRNA HULC stimulates the EMT process and VM in human GBM, and may be a therapeutic target for intervention of GBM.

LncRNA HULC stimulates the EMT process and VM in human GBM, and may be a therapeutic target for intervention of GBM.

The objective of this systematic review was to describe studies that report on whether surface characteristics such as electrostatic charge, surface free energy, and surface topography promote influence on bacterial adhesion on ceramic surfaces.

Searches in the SCOPUS, PubMed/Medline, Web of Science, EMBASE, and Google Scholar databases were performed between December 2020 and January 2021 and updated in March 2021. In addition, a manual search of reference lists from relevant retrieved articles was performed. The criteria included studies that evaluated ceramic surfaces, which described factors such as surface free energy, electrostatic charges, roughness, zeta potential, and their relationship with bacteria.

Database search resulted in 348 papers. Of the 24 studies selected for full reading, 17 articles remained in this systematic review. Another five studies were found in references of articles included, totaling 22 studies. read more These had a high heterogeneity making it difficult to perform statistical analysis, so a descriptive analysis was performed.

For dental ceramics, not enough results were found to demonstrate the influence of the electrostatic condition, and its relationship with bacterial adhesion. However, studies of this review show that there is a correlation between bacterial adhesion, surface free energy, and topography.

The knowledge of ceramics with repulsive physical-chemical interactions would allow an environment suggestive of non-adhesion of pathogenic biofilm.

The knowledge of ceramics with repulsive physical-chemical interactions would allow an environment suggestive of non-adhesion of pathogenic biofilm.Pulmonary tuberculosis (PTB) is a major global public health problem. The purpose of this study was to find biomarkers that can be used to diagnose tuberculosis. We used four NCBI GEO data sets to conduct analysis. Among the four data sets, GSE139825 is lung tissue microarray, and GSE83456, GSE19491 and GSE50834 are blood microarray. The differential genes of GSE139825 and GSE83456 were 68 and 226, and intersection genes were 11. Gene ontology (GO) analyses of 11 intersection genes revealed that the changes were mostly enriched in regulation of leucocyte cell-cell adhesion and regulation of T-cell activation. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs revealed that the host response in TB strongly involves cytokine-cytokine receptor interactions and folate biosynthesis. In order to further narrow the range of biomarkers, we used protein-protein interaction to establish a hub gene network of two data sets and a network of 11 candidate genes. Eventually, IRF1 was selected as a biomarker. As validation, IRF1 levels were shown to be up-regulated in patients with TB relative to healthy controls in data sets GSE19491 and GSE50834. Additionally, IRF1 levels were measured in the new patient samples using ELISA. IRF1 was seen to be significantly up-regulated in patients with TB compared with healthy controls with an AUC of 0.801. These results collectively indicate that IRF1 could serve as a new biomarker for the diagnosis of pulmonary tuberculosis.Anemia treatment is crucial in the management of dialyzed patients. Although Hb and serum ferritin levels are commonly used as indicators for treatment, these values change over time due to changes in policy, drugs for treating anemia, and target levels suggested by clinical guidelines. To clarify long-term changes in anemia-related parameters in Japan, we extracted annual patient data from a newly developed web-based system by the Japanese Society for Dialysis Therapy, the Web-based Analysis of Dialysis Data Archives system. Hb levels gradually increased from 2008 to 2019. Serum ferritin levels and transferrin saturation tended to increase between 2012 and 2019. Although these changes were found in all dialyzed patients, these were more pronounced in patients on peritoneal dialysis. We believe that our results can contribute to a better understanding of the results of clinical studies assessing the effects of treatment for anemia on clinical outcomes among dialyzed patients.The synthesis of porous ionic polymers (PIPs) via the Menshutkin reaction is intriguing because the reaction works smoothly in catalyst-free condition with 100 % atom utilization. However, the rotation of methane site, nonrigid knots, and charge interaction all may cause collapses of the channel, which is detrimental to the synthesis PIP in solid-state conditions. In this work, an inorganic salt (NaBr, NaCl pollution-free and easy to recycle) was rationally chosen as the hard template and effectively prevented the intermolecular packing. Moreover, the increased surface area dramatically promoted the catalytic activity of PIP for cyclic carbonate synthesis. This work provides a green and efficient strategy to construct PIPs via the Menshutkin reaction.To explore the role of suppressor of cytokine signaling-3 (SOCS-3) in mesangial proliferative glomerulonephritis (MsPGN). SOCS-3 expression in kidney tissues from MsPGN patients was detected using immunohistochemistry. Double immunofluorescence staining was performed to investigate the localization of SOCS-3 with α-SMA in glomeruli. Heminephrectomized wild-type (WT) and SOCS-3-/- (KO) mice were injected with Habu-snake venom (HSV) to establish MsPGN models, and renal function were compared. Simultaneously, immunofluorescence, periodic acid-Schiff staining, Picrosirius red staining, as well as immunohistochemistry for PCNA, MAC-2 and type IV collagen in glomeruli were performed. In addition, primary mouse renal mesangial cells and SV40 MES-13 cells were transfected with SOCS-3 siRNA or SOCS-3 lentiviral activation particles, followed by EdU assay, flow cytometry, quantitative reverse transcription-polymerase chain reaction, and Western blotting. Mesangial SOCS-3 expression was enhanced in glomeruli of MsPGN patients, and SOCS-3 was well co-localized with activated α-SMA.