Activity

Immune checkpoint inhibitors (ICIs) have shown significant efficacy in various solid tumors, but only a small subgroup of patients benefit from them because of immune resistance. Oncorine (formerly H101), a recombinant human adenovirus type 5, has direct anticancer properties and enhances cell-mediated immune responses. At present, few studies on the role of Oncorine in reversing resistance to ICIs have been reported. Here, we present a case with recurrent non-small cell lung cancer (NSCLC). The patient developed resistance to nivolumab therapy. After trying immunotherapy plus chemotherapy or antiangiogenesis therapy, the patient only obtained a transient response. The patient then received experimental treatment with Oncorine together with nivolumab and anlotinib. She experienced symptomatic improvement with a performance status score of 1, and achieved stable disease despite partial lung tissue necrosis. This was a successful exploration of oncolytic viruses reversing immune resistance.Molybdenum oxycarbide clusters are novel nanomaterials that exhibit attractive catalytic activity; however, the methods for their production are currently very restrictive. This work represents a new strategy for the creation of near-subnanometer size molybdenum oxycarbide clusters on multilayer graphene. To adsorb Mo-based polyoxometalates of the type [PMo12 O40 ]3- as a precursor for Mo oxycarbide clusters, the novel tripodal-phenyl cation N,N,N-tri(4-phenylbutyl)-N-methylammonium ([TPBMA]+ ) is synthesized. [TPBMA]+ exhibits superior adsorption on multilayer graphene compared to commercially available cations such as tetrabutylammonium ([nBu4 N]+ ) and tetraphenylphosphonium ([PPh4 ]+ ). Using [TPBMA]+ as an anchor, highly dispersed precursor clusters (diameter 1.0 ± 0.2 nm) supported on multilayer graphene are obtained, as confirmed by high-resolution scanning transmission electron microscopy. Remarkably, this new material achieves the catalytic reduction of CO2 to selectively produce CO (≈99.9%) via the reverse water-gas-shift reaction, by applying carbothermal hydrogen reduction to generate Mo oxycarbide clusters in situ.

The osteoarthritis (OA)-associated single nucleotide polymorphism (SNP) rs11583641 is located within COLGALT2, encoding a post-translational modifier of collagen. In cartilage, the SNP genotype correlates with DNA methylation (DNAm) within a putative enhancer. We used patient samples and a chondrocyte cell model to characterise the mechanistic relationship between rs11583641, the putative enhancer, and COLGALT2 expression.

Nucleic acids were extracted from patient cartilage (n=137). Samples were genotyped and DNAm was quantified at 12 CpGs by pyrosequencing. The putative enhancer was deleted in Tc28a2 chondrocytes using CRISPR-Cas9, and the impact upon nearby gene expression was determined by RT-qPCR. Targeted modulation of the epigenome using dCas9-DNMT3a and -TET1 constructs allowed investigation of a causal relationship between DNAm and enhancer activity.

Genotype at rs11583641 correlated with DNAm at three CpGs, with the OA effect allele, C, corresponding with reduced methylation. Deletion of the enompelling osteoarthritis susceptibility target.Active catalysts for HER/HOR are crucial to develop hydrogen-based renewable technologies. The interface of hetero-nanostructures can integrate different components into a single synergistic hybrid with high activity. Here, the synthesis of PdO-RuO2 -C with abundant interfaces/defects was achieved for the hydrogen evolution reaction (HER) and hydrogen oxidation reaction (HOR). It exhibited a current density of 10 mA cm-2 at 44 mV with a Tafel slope of 34 mV dec-1 in 1 m KOH. The HER mass activity was 3 times higher in base and comparable to Pt/C in acid. The stability test confirmed high HER stability. The catalyst also exhibited excellent HOR activity in both media; in alkaline HOR it outperformed Pt/C. The exchange current density i0,m of PdO-RuO2 /C was 522 mA mg-1 in base, which is 58 and 3.4 times higher than those of Pd/C and Pt/C. The HOR activity of PdO-RuO2 /C was 22 and 300 times higher than those of PdO/C in acid and base. Improvement of HER/HOR kinetics in different alkaline electrolytes was observed in the order K+ less then Na+ less then Li+ , and increase of HER as well decrease of HOR kinetics was observed with increasing Li+ concentration. It was proposed that OHad -M+ -(H2 O)x in the double-layer region could influence HER/HOR activity in base. Based on the hard and soft acid and base (HSAB) theory, the OHads -M+ -(H2 O)x could help to remove more OHads into the bulk, leading to increase in HER/HOR activity in alkaline electrolyte (K+ less then Na+ less then Li+ ) and increasing the HER with increasing Li+ concentration. The decrease of HOR activity of PdO-RuO2 /C with increasing M+ was due to M+ -induced OHads destabilization through the bifunctional mechanism. The high HER/HOR activity of PdO-RuO2 /C could be attributed, among other factors, to interface engineering and strong synergistic interaction. Nazartinib order This work provides an opportunity to design oxide-based catalysts for renewable energy technologies.

A lack of standardisation of documentation accompanying older people when transferring from residential to acute care is common and this may result in gaps in information and in care for older people. In Ireland, this lack of standardisation prompted the development of an evidence based national transfer document.

To pilot a new national transfer document for use when transferring older people from residential to acute care and obtain the perceptions of its use from staff in residential and acute care settings.

This was a pre- and post-study design using purposive sampling following the STROBE guidelines. The pilot was conducted in 26 sites providing residential care and three university hospitals providing acute care. Pre-pilot questionnaires focused on current documentation and were distributed to staff in residential care (n=875). A pilot of the new paper-based transfer document was then conducted over three months and post-pilot questionnaires distributed to staff from both residential and acute care settings (n=1085).