Activity

Nanotechnology is a cumbersome field used in industrial, medical and environmental applications. Abundant information regarding the genotoxicity of gold nanoparticles is available, but limited information is available about the genotoxicity of enzymes and bioconjugates of gold nanoparticles from microbial sources. The present investigation reveals the genotoxicity of bioconjugate of tyrosinase and gold nanoparticles from Streptomyces sp.

Tyrosinase and gold nanoparticles were isolated from Streptomyces tuirus DBZ39 and employed for the development of bioconjugates by the flocculation assay method. Methyl thiazole (MTT) assay, transmission electron microscopy, Dynamic Light Scattering (DLS) and UV-vis absorption spectroscopy were used for the investigation.

Bioconjugate showed a significant genotoxic effect at a concentration of 18 μL and at least 10 μL. Bioconjugate of tyrosinase and gold nanoparticles by Streptomyces tuirus DBZ39 revealed a more expressed genotoxic effect than nanoparticles alone. The efficacy of gold nanoparticles was expected to improve the bonding of tyrosine due to their catalytic properties.

This innovative concept of the application of Tyr-AuNps bioconjugate can be used for the development of powerful weapons in medicinal fields. The introduction of bioconjugates of enzymes and nanoparticles in the field of drug design and cancer imaging will be a fundamental breakthrough in medicine.

This innovative concept of the application of Tyr-AuNps bioconjugate can be used for the development of powerful weapons in medicinal fields. The introduction of bioconjugates of enzymes and nanoparticles in the field of drug design and cancer imaging will be a fundamental breakthrough in medicine.

Barley straw is a farm by product that may be useful in animal feeding but its use is limited due to its low nutritional value and high fiber content. Therefore, this study aimed to improving the nutritive value of barley straw by bacterial treatment with Condensed Molasses Soluble (CMS) in the ration of Barki lambs.

In Experiment 1, 12 Barki rams, which were divided into 3 equal groups (4 each), were given rations which consisted of 50% CFM+50%, either clover hay (C) or bacterial treated barley straw (T1) or T1+ CMS (T2). In Experiment 2, 10 Barki lambs were divided into 2 groups, one (C) and T2 as descripted in Experiment 1. Data analyzed by using one way ANOVA model.

Digestibility of Dry Matter (DM), Organic Matter (OM), Crude Fiber (CF) and Ether Extract (EE) were insignificantly affected among all rations, while Digestible Crude Protein (DCP) recorded a higher value in T2 compared to T1. Changes in Total Digestible Nutrients (TDN) values were insignificant. Values of DCP were higher for T2 compared to C and T1. The Body Weight Gain (BWG) and Feed Conversion Ratio (FCR) of growing lambs didn’t statistically differ among the groups.

Finally, complete replacement of clover hay by treated barley straw plus CMS may be considered good feeding strategies in the feeding of Barki lambs in Arid and semi-arid regions.

Finally, complete replacement of clover hay by treated barley straw plus CMS may be considered good feeding strategies in the feeding of Barki lambs in Arid and semi-arid regions.

Soil water mainly derived from rainfall is an important limiting factor to increase crop production. Zegocractin Water balance is used to see the availability of soil water for plants at a certain time. This study aims to determine water supplies, water use and potential water loss using plant water balance and designing cropping patterns on dryland in Ranomee to subdistrict.

The analysis of soil water’s availability is presented through monthly graphs from January to December in climatological terms. The data used in this analysis are the average amount of monthly rainfall and the average monthly temperature in 2016-2018 from the Meteorological, Climatological and Geophysical Agency in South Konawe Regency representing the study area, Ranomeeto subdistrict and the land area of this district. Ranomeeto Subdistrict of South Konawe has a 7 month growth period, from the 2nd November to the 2nd July.

Analysis of the contribution of surface water to soil water content in the plant root zone shows that the Ranomeeto subdistrict of Konawe Selatan Regency has seven months-long growth period available from the second decade of November to the second decade of July.

The planting time in the Ranomeeto subdistrict is in two periods of time, which is in November of decade three to February of decade three (during the rainy season) and May of decade one to July of decade one (during dry season).

The planting time in the Ranomeeto subdistrict is in two periods of time, which is in November of decade three to February of decade three (during the rainy season) and May of decade one to July of decade one (during dry season).

The increase of antibiotic-resistant bacteria is a problem for global health that needs to find new antibiotic drugs. The plant is the potential source of antibiotic substances that important to solve the antibiotic-resistant bacteria. This study was aimed to evaluate the antibacterial activity of Zamioculcas zamiifolia stem extracts against nine human pathogenic bacteria.

Z. zamiifolia stems were extracted with five extraction solvents. The screening of antibacterial activity of stem extract was measured using agar disc diffusion assay. The Minimal Inhibition Concentration (MIC) and Minimal Bactericidal Concentration (MBC) values of extracts were determined using the broth microdilution assay and colorimetric assay.

The results indicated that the lowest MIC value of 0.09 mg mL-1 against Staphylococcus aureus TISTR 1466 was obtained from hexane extraction. The lowest MBCs value of 1.56 mg mL-1 against Bacillus cereus TISTR 2373, Listeria spp. and Escherichia coli TISTR 527 were obtained from ethanol and methanol extractions.

The ethanolic and methanolic stem extracts of Z. zamiifolia demonstrated the highest anti-human pathogenic bacterial activity. This is the first report to demonstrate the high potential of antibacterial substance from Z. zamiifolia stem extracts, which can be developed further as a natural drug for treating bacterial infectious diseases.

The ethanolic and methanolic stem extracts of Z. zamiifolia demonstrated the highest anti-human pathogenic bacterial activity. This is the first report to demonstrate the high potential of antibacterial substance from Z. zamiifolia stem extracts, which can be developed further as a natural drug for treating bacterial infectious diseases.