Activity

The tick Haemaphysalis flava (Acari, Ixodidae) is an obligatory blood-feeding ectoparasite of the giant panda and is also a vector for transmission of pathogenic microorganisms. In this study, the complete mitochondrial genome of this tick was sequenced through Illumina sequencing technology. The genome was 14,699 bp in length and encoded 37 genes including 13 protein-coding genes, 22 transfer RNAs and two ribosomal RNAs. Phylogeny revealed that three isolates of H. flava, regardless of host origins and locations, clustered together and formed a monophyletic relationship with Haemaphysalis japonica, supporting their species validity among the genus Haemaphysalis. These cumulative mitochondrial DNA data provides insights into phylogenetic studies among Haemaphysalis ticks.Philadelphus pekinensis Rupr. is a common perennial deciduous shrub distributed in temperate China. Here, we report the complete chloroplast genome of P. pekinensis. The cp genome is totally 157,308 bp in length, including large single-copy (LSC) region of 86,457 bp, small single-copy (SSC) region of 18,735 bp, and two separated inverted regions (IRs) of 26,058 bp, respectively. It contains 134 genes, including 85 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. The overall GC content is 43.1%. The phylogenetic analysis reveals the monophyly of P. pekinensis and P. calvescens, which is more related with Carpenteria californica than other species in the Hydrangeaceae family.The length of Lelecella limenitoides complete mitogenome was 15,203 bp and contained the typical gene arrangement, base composition, and codon usage found in other related species. The overall base composition exhibited obvious anti-G (7.5%) and AT bias (81.6%). The initiation codons of all PCGs were typical ATN (ATA/ATG/ATT), and the termination codons were TAA, TAG, or incomplete stop codon T-. All tRNAs could be folded into typical cloverleaf secondary structures, except tRNASer (AGN). Phylogenetic analysis showed that L. limenitoides was clustered with the clade of Sasakia, Euripus, and Apatura.The nearly complete mitochondrial genome (mitogenome) of Teredorus nigropennis was determined and analyzed. This mitogenome was 14,652 bp in size and encoded 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes. The most common start codon is ATN, the most common termination codon is TAA and two genes have incomplete termination codon T (TA). The overall nucleotide composition was 45.2% of A, 10.2% of G, 28.6% of T, and 16.1% of C. The data will increase the basic information of Tetrigidae phylogenetic research and can help to better understand the phylogenetic status of T. SEL120 CDK inhibitor nigropennis in Tetrigiodea.In the present study, we determined complete mitogenome of Brontispa longissima, the first for the tribe Cryptonychini. This mitochondrial genome contains 15,696 bp, with an A+T content of 72.2% (GenBank accession no. MN052901). All of the 22 tRNA genes displayed a typical clover-leaf structure, with the exception of tRNASer (TCT). 12 PCGs were initiated by ATN codons, and ND1 started with TTG. Eight PCGs used the typical stop codon ‘TAA’ and ‘TGA’, while five PCGs terminated with incomplete stop codons “T”. Phylogenetic analyses performed using the mitogenomes for the B. longissima and other seven species from subfamily Cassidinae.In this study, we reported a complete mitochondrial genome of Chinese Serow from Guiyang. It has a circular genome of 16,442 bp including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA genes, and a control region. In the phylogenetic tree, we found it clustered tightly with a sequence with a distinct white forehead and white face outlook in the genus Capricornis, which indicated there are a certain number of this species.Due to the multiple causes, the population of roe deer has declined significantly. In this study, we analyzed the complete mitogenome of Capreolus pygargus bedfordi, whose genome was 16,357 bp long. There were 13 protein-coding genes (PCG), two ribosomal RNA genes (12S rRNA and 16S rRNA), 22 transfer RNA genes, and one control region. Nine PCGs started with ATG, while NAD2, NAD3, and NAD5 genes commenced with ATA, and ND4L began with GTG. ND6 and eight tRNA genes were encoded on the L-strand. These results provide newer molecular information, which contribute to its molecular and phylogenetic studies, and genetic diversity conservation.We, at first, fully characterized the complete chloroplast genome of the woody bamboo Acidosasa gigantea using genome skimming and focused on comparative analyses among Acidosasa and Indosasa. This newly sequenced chloroplast genome (GenBank NO. MN917206) is a typical circular structure with 139,711 bp in length and comprises of an 83,295 bp large single-copy (LSC) region, a 12,824 bp small single-copy (SSC) region, and a pair of 21,796 bp inverted repeats (IRs). The GC content of A. gigantea is 38.9% and the sequences contained 132 unique genes were successfully annotated, including 39 tRNA genes, 8 rRNA genes, and 85 protein-coding genes. link2 Phylogenetic analysis shows that A. gigantea is highly clustered in the Arundinaria clade (clade VI) of Arundinarieae, a sister of the clade of Acidosasa purpurea and was not clustered on the same branch as Indosasa sinica. Therefore, it is more accurate to classify A. link3 gigantea into Acidosasa.The complete mitochondrial genome of the Oreolalax major (17,786 bp long) was obtained in this study. It includes 13 protein-coding genes (PCG), two ribosomal RNA (rRNA) genes, and 23 transfer RNA (tRNA) genes (GenBank accession number MN803320). The phylogenetic tree indicates that the O. major is closely related to the O. xiangchengensis.Ferret-badger (Melogale moschata) is a widely distributed and rarely studied species. Therefore, we sequenced its complete mitochondrial genome, which is circular in shape with 16,511 bp length and have a structure typical to mammals. There is 98.67% similarity when compared with the reported sequence (HM106328). It contained 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control regions (D-loop). This study can provide novel molecular information and genetic diversity of ferret-badger.Red-and-white giant flying squirrel (Petaurista alborufus) is a widely distributed but rarely studied species. Here, we sequenced the complete mitochondrial genome about it, which is a circular genome of 16,511 bp and have a typical structure with mammals. The base composition shows higher composition of A\T. There are 61 variations and eight base deletions between two sequences when compared with the reported sequence (JQ743657). In this study, we obtain a new mitochondrial genome sequence of red-and-white giant flying squirrel, which provide novel molecular information that could prove fundamental to our understanding of diversity research and phylogenetic structure.Lilium pumilum DC. is a useful plant species not only for its showy flowers but also for its edible and medicinal values. Here we report on the complete chloroplast genome sequence of L. pumilum. The chloroplast genome is 152,573 bp in size and includes two inverted repeat regions of 52,984 bp, which is separated by a large single-copy region of 82,009 bp and a small single copy region of 17,580 bp. A total of 130 genes were predicted, including 38 tRNA, 8 rRNA, and 84 protein-coding genes. Phylogenetic analysis placed L. pumilum under the family Liliaceae.Hemerocallis citrina (Asphodelaceae) has been wildly cultivated as ornamental and medicinal plant. Here, we reported the first chloroplast genome sequence of H. citrina. The chloroplast genome size is 156,088 bp with GC content of 37.3%, including a large single-copy (LSC) of 84,843 bp, a small single-copy (SSC) of 18,507 bp, and a pair of 26,369 bp IR(inverted repeat) regions. A total of 133 genes were annotated including 87 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. The phylogenetic analysis revealed that H. citrina belongs to the Hemerocallis genus in Asphodelaceae family.We sequenced and annotated the species of Sphaeronemoura elephas which represents the first record for continental China from Jiangxi Province in this study to provide mitochondrial genome data for future studies. The entire mitochondrial genome of S. elephas harbored 37 typical code genes and one control region with 15,846 bp in length. The A + T account of total nucleotide, PCGs, tRNAs, rRNAs and control region were 67.1, 64.5, 70.5, 71.0, 82.4%, respectively and the A + T content was the highest in control region. The start codon of all PCGs used ATN except ND5 and ND1 started with GTG and TTG. Eleven PCGs used typical terminal codon TAA or TAG while the COII and ND5 stopped with the single T. Based on 13 PCGs by using Bayesian (BI) and maximum-likelihood (ML) methods, we found that the genus Sphaeronemoura and Mesonemoura were sister groups and the species of Amphinemurinae was monophyletic group.The first complete chloroplast genome (cpDNA) sequence of Litsea garrettii was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 154,011 bp in length, contains a large single-copy region (LSC) of 93,697 bp and a small single-copy region (SSC) of 18,826 bp, which were separated by a pair of inverted repeat (IR) regions of 20,744 bp. The genome contains 127 genes, including 82 protein-coding genes, 8 ribosomal RNA genes, and 36 transfer RNA genes. Further phylogenomic analysis showed that L. garrettii and Parasassafras confertiflorum clustered in a clade in Lauraceae family.In this study, high-throughput Illumina sequencing was employed to assemble the complete mitochondrial genome of the Meiren yak (Bos grunniens), a local yak breed from Gansu Province, China. The mitochondrial genome is 16,321 bp long with an A + T-biased nucleotide composition and harbors 13 protein-coding, 22 Trna, and 2 rRNA genes, and a noncoding control region. The mitogenomic organization and codon usage are highly similar to those of previously published congeneric mitochondrial genomes. Bayesian phylogenetic analysis indicates that Meiren yak is most closely related to nine other yak breeds (incl. Datong, Huanhu, Pali, Pamir, Polled, Qilian, Seron, Sunan, and Tianjun yaks).The meerkat, Suricata suricatta, is a highly social member of the mongoose family (Herpestidae) and the only extant species of the genus Suricata. We present the first complete mitochondrial genome of the meerkat, assembled with a seed-and-extend algorithm using three closely related species as references. Phylogenetic analyses using 22 mitochondrial genome sequences confirm the position of meerkat within the Herpestidae family and the Feliformia, a suborder of Carnivora, with high support values. This position is in good agreement with formerly conducted studies based on a small number of mitochondrial and nuclear gene fragments. Our complete mitochondrial genome represents a valuable resource for further phylogenetic studies, especially of the underrepresented members of the Herpestidae family.The first complete mitochondrial genome (mt) of Paraprenanthes diversifolia (Vaniot) N. Kilian (Cichorieae; Asteraceae) was sequenced and successfully assembled in this study. The full length of the mt genome is 360,751 bp, containing 73 genes (33 protein-coding genes, 29 tRNA genes, 6 rRNA genes, and 5 protein-coding genes containing internal stop codons). There are two pairs of long (over 1000 bp) repeat regions in the mt genome of P. diversifolia. The phylogenetic analysis indicated that P. diversifolia has a close relationship with other Lactucinae species.