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An improved pretreatment approach of human breast tissue is demonstrated for subsequent analysis of seven parabens including methyl paraben (MPB), ethyl paraben (EPB), propyl paraben (PPB), butyl paraben (BPB), isopropyl paraben (iPPB), isobutyl paraben (iBPB), and benzyl paraben (BzPB). Specifically, a well-designed homogenization procedure, conjugated with an optimized fabric phase sorptive extraction (FPSE) protocol, resulted in a carefully outlined sample preparation process as part of a green, simple, sensitive, economical and fast HPLC-PDA analytical method in agreement with Green Analytical Chemistry (GAC) demands. Among all tested FPSE membranes, the highest extraction efficiency was achieved by employing sol-gel poly(tetrahydrofuran) (sol-gel PTHF) coating on 100% cotton cellulose fabric that represents a medium polarity microextraction device, which combined the advanced material characteristics of sol-gel sorbent and the rich surface chemistry of an inherent porous cellulose fabric substrate. The cated to be suitable and efficient for future epidemiological and toxicological studies.Chelators, capable of creating soluble complexes with metals, may disrupt the natural speciation of metals in environmental matrices. Detection of environmental speciation of such complexes has remained challenging as obtaining the precise inherent nature of metal-chelator complexes is difficult by using routine techniques. KT 474 Herein, we report a rapid and sensitive technique for the speciation analysis of complexes of five metal ions (Ni, Pb, Co, Fe and Ca) with two aminopolycarboxylate chelator variants, namely, EDTA (ethylenediaminetetraacetic acid) and EDDS (ethylenediamine-N,N’-disuccinic acid), including the simultaneous quantification of those complexes. EDTA is characterized as environmentally persistent among the chelators used in the current work whereas EDDS is biodegradable. The speciation analysis was performed using ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The separation was achieved by using hydrophilic interaction liquid chromatographic column. The effect of various operating parameters on analytes such as mobile-phase composition, buffer concentrations and pH, sample diluents, sample injection volume, and column temperature on the peak shape and sensitivity were systematically optimized. The dilution was the only requirement for preparing the samples for analysis. The average relative uncertainty was 2.4% with the average precision (as RSD, n= 7) of 3.5%. For the metal-EDTA complexes, LOD range was 3 to 76 nmol L-1 with satisfactory recovery from a simulated mix matrix (recovery 79-97%) and river water by standard addition (recovery 82-94%). For metal-EDDS complexes, LOD range was 66 to 293 nmol L-1 with recovery from a simulated mix matrix (recovery 56-97%) and river water by standard addition (recovery 61-91%). The proposed method will be applicable in speciation analysis and simultaneous detection of metal-chelator complexes from environmental samples.Novel core shell structured magnetic covalent organic frameworks were synthesized at room temperature and first applied in water samples for the enrichment of trace polybrominated diphenyl ethers (PBDEs) through magnetic solid-phase extraction. The prepared materials were characterized through transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, vibrating sample magnetometer and X-ray photoelectron spectroscopy. During adsorption, the parameters affecting extraction and desorption efficiency were further optimized. Combined gas chromatography and mass spectrometry (GC/MS) revealed that high enrichment factors (275-292), low limits of detection (0.12-0.38 ng·L-1), wide linear ranges (0.5-1000 ng·L-1), and good reproducibility (intra-day 1.40%-4.31% and inter-day 5.14%-9.12%) were obtained under optimal conditions. The method successfully detected PBDEs in different water samples.

In many countries, various continuity models of midwifery care arrangements have been developed to benefit women and babies. In Sweden, such models are rare.

To evaluate two on-call schedules for enabling continuity of midwifery care during labour and birth, in a rural area of Sweden.

A participatory action research project where the project was discussed, planned and implemented in collaboration between researchers, midwives and the project leader, and refined during the project period. Questionnaires were collected from participating women, in mid pregnancy and two months after birth.

One of the models resulted in a higher degree of continuity, especially for women with fear of birth. Having a known midwife was associated with higher satisfaction in the medical (aOR 2.02 (95% CI 1.14-4.22) and the emotional (aOR 2.05; 1.09-3.86) aspects of intrapartum care, regardless of the model.

This study presented and evaluated two models of continuity with different on-call schedules and different possibilities for women to have access to a known midwife during labour and birth. Women were satisfied with the intrapartum care, and those who had had a known midwife were the most satisfied. Introducing a new model of care in a rural area where the labour ward recently closed challenged both the midwives’ working conditions and women’s access to evidence-based care.

This study presented and evaluated two models of continuity with different on-call schedules and different possibilities for women to have access to a known midwife during labour and birth. Women were satisfied with the intrapartum care, and those who had had a known midwife were the most satisfied. Introducing a new model of care in a rural area where the labour ward recently closed challenged both the midwives’ working conditions and women’s access to evidence-based care.Increasing evidence shows that inflammation plays critical roles in the tumorigenesis of bladder cancer. Fibroblast growth factor 12 (FGF12), a kind of inflammatory cytokine, is located in the region of 3q28 that has been demonstrated to be a bladder cancer risk locus by genome wide association study (GWAS). In this study, we aimed to investigate the association of GWAS signal rs710521 and rs884309 and rs1464938 in the promoter of FGF12 with the risk of bladder transitional cell carcinoma (TCC). The polymorphisms were analyzed by using a Taqman assay in 331 TCC patients and 516 age-, gender-, and ethnicity-matched controls. The expression levels of FGF12 mRNA were examined in TCC and non-cancerous normal tissues by using quantitative real-time PCR and the luciferase activity was determined by using the Dual-Luciferase Assay System. The rs1464938 AA genotype and A allele were associated with a significantly increased risk of TCC (AA vs. GG adjusted OR = 2.54, 95% CI, 1.49-4.35, P less then 0.001; AA vs. AG/GG adjusted OR = 2.