Activity

Once the irradiation was limited, STEM-EELS investigation was conduct using DualEELS on the Si and Al L2,3 and OK edge fines structures. The interface was found to be composed of a-SiOx and non-stoichiometric aluminum silicate with a predominance of tetrahedrally coordinated Al in its first layer.Infection with HIV-1 remains uncurable due to reservoirs of latently infected cells. Any potential cure for HIV will require a mechanism to identify and target these cells in vivo. We created a panel of Jurkat cell lines latently infected with the HIV DuoFlo virus to identify candidate biomarkers of latency. SWATH mass spectrometry was used to compare the membrane proteomes of one of the cell lines to parental Jurkat cells. Several candidate proteins with significantly altered expression were identified. The differential expression of several candidates was validated in multiple latently infected cell lines. Three factors (LAG-3, CD147,CD231) were altered across numerous cell lines, but the expression of most candidate biomarkers was variable. These results confirm that phenotypic differences in latently infected cells exists and identify additional novel biomarkers. The variable expression of biomarkers across different cell clones suggests universal antigen-based detection of latently infected cells may require a multiplex approach.Kaposi’s sarcoma-associated herpesvirus is a human rhadinovirus of the gammaherpesvirus sub-family. Although herpesviruses are well-studied models of capsid formation and its processes, those of KSHV remain unknown. KSHV ORF17 encoding the viral protease precursor (ORF17-prePR) is thought to contribute to capsid formation; however, functional information is largely unknown. Here, we evaluated the role of ORF17 during capsid formation by generating ORF17-deficient and ORF17 protease-dead KSHV. Both mutants showed a decrease in viral production but not DNA replication. ORF17 R-mut, with a point-mutation at the restriction or release site (R-site) by which ORF17-prePR can be functionally cleaved into a protease (ORF17-PR) and an assembly region (ORF17-pAP/-AP), failed to play a role in viral production. Furthermore, wild type KSHV produced a mature capsid, whereas ORF17-deficient and protease-dead KSHV produced a B-capsid, (i.e., a closed body possessing a circular inner structure). Therefore, ORF17 and its protease function are essential for appropriate capsid maturation.Although traffic crashes involving buses are less frequent than those involving other vehicle types, the consequences of bus crashes are high due to the potential for multiple injuries and casualties. As driver error is a primary factor affecting bus crashes, driver safety education is one of the main countermeasures used to mitigate crash risk. In China, however, safety education is not as focused as it should be, largely due to the limited research identifying the specific driver behaviors, and potential influences on those behaviors, that are correlated with crashes. The aim of this study is, therefore, to explore the fleet- and driver-level risk factors underlying bus drivers’ self-reported crash involvement, including analyzing the effect of psychological distress on the most influential driver-level factors. A survey was conducted of 725 drivers from a large Shanghai bus company, and a random-effects two-level logit model was developed to integrate fleet and individual variables. Results showed that 1) the fleet-level safety climate explained about 8.5% of the model’s variance, indicating it was a valid predictor of self-reported crash involvement; 2) the driver-level factors of drivers’ age, seniority, marital status, positive behavior, and driving anger influenced drivers’ self-reported crash involvement, but ordinary violations, lapses, aggressive violations, and insomnia were the most influential variables; 3) psychological distress appeared to associate with the high frequency of risky driving behavior and the high severity of driving anger. This study’s findings will help bus companies to give more attention to their safety climate and implement more targeted improvements to their driver safety education programs.

Traditionally, a first step toward independence for a North American youth has been to obtain a driver’s licence. Licensure can be associated with freedom and independence to provide teens opportunities to participate in conventional and health-enhancing behaviours, such as school, work, sporting events and other social activities, and/or provide opportunities to engage in unconventional, risk taking and health compromising behaviours. Yet, recent trends show that youth are delaying getting their licence.

The purpose of this study was to use the constructs of conventional and unconventional behaviours identified in Problem Behaviour Theory (PBT) under the behaviour system to examine whether driver licensure status in youth was associated with conventional, health-enhancing behaviours or unconventional, health compromising, risky behaviours.

Data were derived from the 2015 and 2017 cycles of the Ontario Student Drug Use Survey (OSDUHS). Analyses are based on a subsample of students who were at least 16 yntional behaviours, as well as problem behaviours associated with alcohol use.

In agreement with Problem Behaviour Theory, the results show that youth who obtain their licence show limited problematic lifestyle or ‘proneness’ (Gohari, 2019). Alcohol use and binge drinking were significantly associated with youth obtaining a driver’s licence but hazardous drinking was not. selleck products This study suggests that a driver’s licence for youth could both control but also instigate unconventional behaviours as identified by PBT. A driver’s licence could also provide opportunities for conventional behaviours associated with education and school connectedness. However, the independence provided by a driver’s licence could offer youth unsupervised social opportunities to drink and binge drink that may be health-compromising. Thus, based on PBT, licensure status is associated with both conventional behaviours, as well as problem behaviours associated with alcohol use.