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Coral reefs face multiple threats, including climate change, agricultural runoff, shipping activities, coastal development, and chemical pollutants. Irgarol 1051, a PSII herbicide, has been used as an antifouling booster since the previously used antibiofouling agent tributyltin (TBT) was banned worldwide. Although the mechanisms through which elevated temperatures cause coral bleaching have been reported, it remains unclear how PSII herbicides cause bleaching. Thus, in this study, we investigated the transcriptomes of Acropora tenuis and its symbiotic dinoflagellates by RNA-sequencing (RNA-Seq) to elucidate the molecular mechanisms underlying Irgarol-induced bleaching. Coral exposure to 10 μg/L Irgarol for 7 d affected coral body colour, specifically by an increase in their red, green, and blue (RGB) values; however, no such effect was observed in corals exposed to 1 μg/L Irgarol. RNA-Seq revealed the differentially expressed genes (DEGs) in corals and symbiotic dinoflagellates following Irgarol exposure. Cog the bleaching effect of PSII herbicides, such as Irgarol, on corals and symbiotic dinoflagellates.Sampling of microbial biomass is crucial for understanding and controlling remediation processes ongoing at contaminated sites in general, particularly when molecular genetic analyses are employed. In this study, fiber-based carriers with a nanofiber layer were developed and tested as a method to sample microbial biomass in groundwater for molecular genetic analysis. Nanofiber carriers, varying in the shape and the linear density of nanofibers, were examined throughout a 27-month monitoring period in groundwater contaminated with benzene, toluene, ethylbenzene and xylene isomers (BTEX), and chlorinated ethenes. The effect of carrier shape and nanofiber layer density on the microbial surface colonization and composition of the microbial biofilm was determined using real-time PCR and next-generation sequencing (NGS) analysis. Differences in microbial community composition between nanofiber carriers, groundwater, and soil samples were also analyzed to assess the applicability of carriers for biomass sampling at contaminated sites. The nanofiber carriers showed their applicability as a sampling tool, particularly because of their easy manipulation that facilitates DNA isolation. The majority of taxa (Proteobacteria, Firmicutes, and Bacteroidetes) present on the carrier surfaces were also detected in the groundwater. Moreover, the microbial community on all nanofiber carriers reflected the changes in the chemical composition of groundwater. Although the carrier characteristics (shape, nanofiber layer) did not substantially influence the microbial community on the carrier surface, the circular and planar carriers with a nanofiber layer displayed faster microbial surface colonization. However, the circular carrier was the most suitable for biomass sampling in groundwater because of its high contact area and because it does not require pre-treatment prior to DNA extraction.Soluble microbial products (SMP), a type of polymers released from microbial metabolism and decay, show great potential for wastewater treatment as bioflocculants; however, biogenic flocculant utilization is currently limited to bacterial SMP. In this study, SMP produced by Phanerochaete chrysosporium BKMF-1767 (SMP-P) was investigated to determine the application potential of fungal SMP. SMP-P exhibited high flocculation activity in kaolin suspension at a dosage range of 0.67-0.84 mg/L with Ca2+ assistance, comparable to that of commercial polyacrylamide. The high molecular weight polysaccharides (2.0 × 106-4.7 × 107 Da) in SMP-P, which enabled flocculation via the bridging mechanism and served as the dominant active constituent, were composed of glucose and arabinose at a molar ratio of 1 0.03, with (1 → 4, 6)-linked glucose as the main backbone and a small proportion of branched structures. They contained hydroxyl and carboxyl, effective functional groups for the flocculation process, and displayed parallel self-orientation behavior in water. Efficient chemical oxygen demand removal was achieved during municipal wastewater treatment using SMP-P as the bioflocculant. This study demonstrates the feasibility of utilizing fugal SMP as bioflocculants and provides guidance for their practical application.This work was aimed to comprehensively evaluate the potential for sustainable development of China’s shale gas industry. It will contribute to the sustainable development of China’s energy and economic. selleck Factors of resource, technology, economy and environment were selected to develop the DPSIR framework evaluation indicators in system for shale gas based on the previous research. Next, The PPFCI (projection pursuit fuzzy clustering model) technique was developed by combining the projection pursuit model with a fuzzy clustering iterative model. So that it can deal with the multi-source, high-dimensional, fuzzy data of the proposed evaluation indicators. And then, the RAGA (accelerated genetic algorithm based on real coding) algorithm was developed to run the PPFCI technique. The results show that core technical capability, investment in projects of prevention of geological disasters, and ecological environment damage indicators were the key factors affecting the sustainability of China’s shale gas industry. The potential for sustainable development of China’s shale gas industry was relatively low. And it was unbalanced in different provinces. The potential for sustainable development of the southwest region was better than the northwest region. Among them, the development of Sichuan was more stable than Chongqing, with a 99% probability of maintaining a stable and sustainable development state, while Chongqing province has a 15%-20% probability to fluctuate towards the poles.In this work two sensitive areas of the Spanish coast located in the Atlantic (Ria de Vigo) and Mediterranean (Mar Menor lagoon) have been studied regarding their contamination by phthalates, organotin compounds and per-polyfluoroalkyl substances (seawater and sediments) in two different campaigns (spring and autumn in 2015). PFAS and OTCs were detected in seawater and sediments at low concentrations (few ng L-1 or ng g-1), whereas PAEs were detected at levels two orders of magnitude higher, particularly in Mar Menor lagoon due to its semi-confined characteristics. However, PAEs and OTCs concentration in sediments were higher in Ría de Vigo than in Mar Menor lagoon as a consequence of the influence of the important urban nuclei and port in that area. The ecological risk assessment revealed that in both areas tributyltin, dibutyltin and diethylphthalate pose a significant risk in sediments, whereas in seawater tributyltin in both areas resulted in a high risk.Drinking water provision has been a constant challenge in the Sahrawi refugee camps, located in the desert near Tindouf (Algeria). The drinking water supply system is itself divided in three zones which pump groundwater from different deep aquifers. It is equipped with reverse osmosis plants and chlorination systems for treating water. The allocation of water supplied to the Saharawi refugees for human consumption in 2016 has been estimated at between 14 and 17 L/person/day on average. This supplied water volume is below recommended standards, and also below the strategic objective of the Sahrawi government (20 L/person/day). Yet the local groundwater resources are huge in comparison with estimated consumption, and hence there is great potential for increasing the supplied volume through effecting improvements in the supply system. The physico-chemical quality of the raw and supplied water between 2006 and 2016 has been assessed according to Algerian standards for human consumption. The raw water of two zones of the supply system presents a very high conductivity and high concentrations of chloride, nitrate, fluoride, sulfate, sodium, calcium, potassium and iodide concentrations of natural origin, which may entail health risks. The treatment of water in a reverse osmosis plant greatly improves its quality and osmosed water met the standards. However, the supply of osmosed and raw water needs to be combined in Zone 1, to avoid an excessive reduction in water volume, and the supplied raw water poses a risk to the health of the refugees. The present study provides an example of a drinking water supply system under extreme drought conditions and in the political and social conditions of a refugee camp. Furthermore, it establishes a reference for supplied water allocation and quality in the Sahrawi refugee camps.Polycyclic aromatic hydrocarbons (PAHs) are toxic airborne pollutants and may cause adverse effects at high level of oxidative stress. Here we hypothesized that individuals with impaired lung function are susceptible to PAHs associated oxidative damage. Hence, we carried out a panel study and conducted four follow-up visits on 40 chronic obstructive pulmonary disease (COPD) patients and 75 healthy controls. Hydroxylated PAHs (OH-PAHs) and malonaldehyde (MDA) were measured in urine as exposure and oxidative stress markers, respectively, which showed significant association in all participants. Quantitatively, a 1-fold increase in OH-PAHs was associated with a 4.1-15.1% elevation of MDA. The association between OH-PAHs and MDA levels became stronger in participants with impaired lung function. For 1% decrease of FEV1/FVC, the increase of MDA associated with a 1-fold increase in OH-PAHs was up to 0.49%, suggesting an increased susceptibility to PAH-induced oxidative damage in individuals with worse lung function. This study observed that impaired lung function modified the association between PAH exposure and oxidative damage, which might accelerate the aggravation of COPD, and therefore highlighted the necessity of protection measures to decrease the additional adverse effects of air pollution exposure. CAPSULE Individuals with worse lung function may be more susceptible to PAH-induced lipid peroxidation.The pervasive spread of microplastics (MPs) and nanoplastics (NPs) has raised significant concerns on their toxicity in both aquatic and terrestrial environments. These polymer-based materials have implications for plants, wildlife and human health, threatening food chain integrity and ultimate ecosystem resilience. An extensive – and growing – body of literature is available on MP- and NP-associated effects, including in a number of aquatic biota, with as yet limited reports in terrestrial environments. Effects range from no detectable, or very low level, biological effects to more severe outcomes such as (but not limited to) increased mortality rates, altered immune and inflammatory responses, oxidative stress, genetic damage and dysmetabolic changes. A well-established exposure route to MPs and NPs involves ingestion with subsequent incorporation into tissues. MP and NP exposures have also been found to lead to genetic damage, including effects related to mitotic anomalies, or to transmissible damage from sperm cells to their offspring, especially in echinoderms. Effects on the proteome, transcriptome and metabolome warrant ad hoc investigations as these integrated “omics” workflows could provide greater insight into molecular pathways of effect. Given their different physical structures, chemical identity and presumably different modes of action, exposure to different types of MPs and NPs may result in different biological effects in biota, thus comparative investigations of different MPs and NPs are required to ascertain the respective effects. Furthermore, research on MP and NP should also consider their ability to act as vectors for other toxicants, and possible outcomes of exposure may even include effects at the community level, thus requiring investigations in mesocosm models.