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Thus, mortality rates of HM patients needing ICU care will decline.

A separate ICU allocated for haematology patients will allow timely and rapid admission of HM patients to ICU. Thus, mortality rates of HM patients needing ICU care will decline.Backgroun/Aim The presented study aimed to evaluate the utility of Magnetic Resonance Angiography in the pediatric population with nutcracker syndrome.

Patients with suggestive clinical symptoms and laboratory findings and got the diagnosis of nutcracker syndrome with Doppler ultrasonography between January 2011-2019 were included in the study. In addition, children who had renal MRA due to hypertension were evaluated as the control group. MRA images of all patients were examined retrospectively by 3 radiologists at different levels of experience, and the superior mesenteric artery angle, aorta-mesenteric distance, left renal vein diameter both in the regions of aorta-mesenteric and renal hilum were recorded.

Forty-five patients diagnosed with nutcracker syndrome were included in the study. The mean age of patients was 12 (4-16) and 30 (67%) were female. As the control group, 25 patients with hypertension who had MRA were included and they had a mean age of 12 (1-18) and 19 (76%) were male. The mean superior mesenteric artery angle was 26.5 ° (16-73 ± 12) in the patient group and 57.8 ° (25-139, ± 33) in the control group (p <0.001); the mean aorta-mesenteric distance was 3.3 mm (1.7-6.5, ± 1.1) in the patient group and 8 mm (3.4-32, ± 5.9) in the control group (p <0.001). MRA measurements of 3 radiologists were consistent with each other.

MRA imaging can be applied as an alternative diagnostic method for Doppler ultrasonography and Multidetector CT examinations by radiologists with different experience levels in pediatric patients with nutcracker syndrome.

MRA imaging can be applied as an alternative diagnostic method for Doppler ultrasonography and Multidetector CT examinations by radiologists with different experience levels in pediatric patients with nutcracker syndrome.

The purpose of this study was to determine the effects of the Supplemental Feeding Tube Device (SFTD) and bottle methods on weight gain, transition to full breastfeeding, breastfeeding success, and duration of discharge in preterm infants.

This randomized controlled trial was conducted with a total of 46 preterm infants including 23 infants in study (SFTD) and control (bottle) groups. An information form, an infant follow-up form for feeding, and LATCH breastfeeding assessment instrument were used to collect the data.

The gestation week of the infants in the study group was 31.22 ± 2.76, and in the control group it was 30.52 ± 2.47. The birth weight of the infants in the study group was 1586.3 ± 525.35 gr and 1506.09 ± 454.77 gr in the control group. The daily weight gain of the infants was 24.09 ± 15.21 gr in the study group and 27.17 ± 17.63 gr in the control group. The infants in the study group (4.70±2.44days) transitioned to full breastfeeding earlier than those in the control group (6.00±4.10days). LATCH 2nd measurement scores were significantly higher in both groups than LATCH 1st measurement scores (p <0.01). Although it was not statistically significant (p> 0.05), the infants in the study group (10.22 ± 5.20days) were discharged earlier than those in the control group (13.48 ± 8.78days).

The SFTD and bottle methods were determined to be similar in terms of daily weight gain, transition to full breastfeeding, breastfeeding success, and duration of hospitalization.

The SFTD and bottle methods were determined to be similar in terms of daily weight gain, transition to full breastfeeding, breastfeeding success, and duration of hospitalization.

The methylotrophic yeast Pichia pastoris is an appealing production host for a variety of recombinant proteins, including biologics. In this sense, various genetic- and non-genetic-based techniques have been implemented to improve the production efficiency of this expression platform. Loss of supression (Los1) encodes a non-essential nuclear tRNA exporter in Saccharomyces cerevisiae, which its deletion extends replicative lifespan. Adenosine disodium triphosphate Herein, a los1-deficient strain of P. pastoris was generated and characterized.

A gene disruption cassette was prepared and transformed into an anti-CD22-expressing strain of P. pastoris. A δ los1 mutant was isolated and confirmed. The drug sensitivity of the mutant was also assessed. The growth pattern and the level of anti-CD22 single-chain variable fragment (scFv) expression were compared between the parent and mutant strains.

The los1 homologue was found to be a non-essential gene in P. pastoris. Furthermore, the susceptibility of los1 deletion strain to protein synthesis inhibitors was altered. This strain showed an approximately 1.85-fold increase in the extracellular level of anti-CD22 scFv (p < 0.05). The maximum concentrations of total proteins secreted by δ los1 and parent strains were 125 mg/L and 68 mg/L, respectively.

The presented data suggest that the targeted disruption of los1 homologue in P. pastoris can result in a higher expression level of our target protein. Findings of this study may improve the current strategies used in optimizing the productivity of recombinant P. pastoris strains.

The presented data suggest that the targeted disruption of los1 homologue in P. pastoris can result in a higher expression level of our target protein. Findings of this study may improve the current strategies used in optimizing the productivity of recombinant P. pastoris strains.Novel medial preserving gap technique (MPGT) focuses on medial compartment stability and allows lateral physiological laxity. This study aimed to compare the MPGT with the measured resection technique (MRT) to determine which provides better postoperative knee stability after posterior-stabilized total knee arthroplasty (PS-TKA). Primary PS-TKA, using either MPGT (n = 65) or MRT (n = 65), was performed in 130 patients with varus knee osteoarthritis. Postoperative knee stabilities at extension and flexion were assessed using varus-valgus stress radiographs and stress epicondylar view, respectively (at 1 month, 6 months, 1 year, and 3 years postoperatively). The distance between the femoral prosthesis and polyethylene insert was measured on each medial and lateral side, defined as the medial joint opening (MJO) and lateral joint opening (LJO), respectively. Decreasing MJO or LJO translated to increasing postoperative stability. The femoral external rotation angle was compared between the two surgical techniques; postoperative knee stability was also compared between the medial and lateral compartments, as well as the surgical techniques.