Activity

gests no significant association of overall or MVA-free survival with ECG parameters reflecting TDR (TpTe, TpTec) in patients with systolic dysfunction after MI and ICD implanted for primary prevention.

This study suggests no significant association of overall or MVA-free survival with ECG parameters reflecting TDR (TpTe, TpTec) in patients with systolic dysfunction after MI and ICD implanted for primary prevention.Introduction Variable power output (VP) is one of the main characteristics of a road cycling mass-start. Tolerating VP during outdoor road cycling highly influences performance. There is a lack of continuous and comprehensive measurements during this power condition. Accordingly, the aim of the present study was to investigate physiological response to VP vs. constant power output (CP) as well as the perceived exertion of these two power conditions, and to investigate if variations in power output which span above lactate threshold (LT), differ from variations below LT. Methods 15 elite competitive cyclists completed three test days, including 1 day of baseline testing and 2 days of main testing, consisting of four bouts of 28 min at two different intensities, “low” at 70% of LT and “high” at 95% of LT, with VP and CP. VP was performed with a 15% fluctuation of the average power output every second minute. Maximal oxygen uptake (VO2), respiratory exchange ratio (RER), heart rate (HR), blood lactate (LA), rating of perceived exertion (RPE), cadence (RPM) and power output (W) were measured. Results At both low and high intensity, the VP condition induced a significantly higher VO2, HR and LA than the CP condition. Whole-bout RPE was similar between power conditions at high intensity. Additionally, at the high intensity, cycling with VP led to a greater increase in LA and lesser increase in RPE compared to cycling with CP. Discussion The results of this study show that, despite considerable differences in the demand during the VP and CP bouts, there are minor differences in the perceptual and physiological response directly following these two power conditions in a cohort of elite competitive cyclists. A practical implication of these findings is that training with VP seems to be a viable alternative to training with CP, at least at high intensity.Hypoxic exercise may represent a novel therapeutic strategy to reduce and prevent obesity through the regulation of lipid metabolism. During hypoxic exercise, the targeting of peroxisome proliferator-activated receptor gamma (PPARγ) by miR-27b has been proposed to be one of the mechanisms involved in the modulation of lipid metabolism. We have previously shown that miR-27b can repress PPARγ and lipid metabolism-associated factors, thereby affecting lipid metabolism during hypoxic exercise in a rat model of obesity. In the current study, we aimed to confirm the role of miR-27b in the regulation of lipid metabolism. First, miR-27b expression was either upregulated or downregulated through the injection of adeno-associated virus (AAV) 9 containing a miR-27b expression cassette or miR-27b-3p inhibitor, respectively, into the right gastrocnemius muscle of obese rats. The rats were then subjected to a 4-week program of hypoxic exercise, and a series of parameters related to lipid metabolism were systematically evalsterol 7 alpha-hydroxylase (CYP7A1) and fatty acid translocase 36 (CD36) levels. Similarly, at the protein level, miR-27b overexpression promoted a decrease in the concentration of PPARγ, whereas miR-27b inhibition led to an increase in PPARγ levels, as well as those of CYP7A1, CD36, ATGL, and LPL. Overall, our results indicated that hypoxic exercise regulates lipid metabolism via the miR-27b/PPARγ pathway and modulates ATGL and LPL expression through inducing their post-transcriptional modifications.Helicoverpa punctigera (native budworm) is an important pest species in crops across Australia. From the third instar onward, this species causes severe damage to crop plants therefore, caterpillars need to be managed at an early stage of their development. In our experiment, we raised H. punctigera on an artificial diet, which included different concentrations of the natural insecticides Spinetoram and Azadirachtin. The survival of the larvae, growth and body mass gain was recorded over 17 days. Only caterpillars raised on lowest toxin concentrations survived and molted successfully to the fifth instar, but had slower growth and body mass gain compared to the insecticide-free control group. Caterpillars fed on higher toxin concentrations never molted to the next instar or died in the first few days. To test how the toxins influence physiological conditions including metabolic rate and water loss, surviving fifth instar larvae were exposed to thermolimit respirometry starting at 25°C following a constant increasing temperature ramping rate of 0.25°C-1, until reaching the critical thermal maxima (CT max ). Caterpillars raised on a non-lethal dose of insecticides had higher metabolic rates and lost more water compared to the control group. Insects that have seem to consume more energy per mg tissue and have a higher water loss at high temperatures. Non-lethal concentrations of insecticides on pest insects physiology may reduce their impact on crops and may enable more targetted insecticide application.Cold weather is one of the biggest challenges in establishing a large-scale microalgae culture facility in temperate regions. In order to develop a strain that is resistant to low temperatures and still maintains high photosynthetic efficiency, transgenic studies have been conducted targeting many genes. Early light-inducible proteins (ELIPs) located in thylakoid membranes are known to protect photosynthetic machinery from various environmental stresses in higher plants. An ELIP homolog was identified from Chlamydomonas reinhardtii and named ELIP3. The role of the gene was analyzed in terms of photosynthetic CO2 assimilation under cold stress. Western blot results showed a significant accumulation of ELIP3 when the cells were exposed to cold stress (4°C). Ruboxistaurin datasheet High light stress alone did not induce the accumulation of the protein. Enhanced expression of ELIP3 helped survival of the cell under photo-oxidative stress. The influx of CO2 to the photobioreactor induced strong accumulation of ELIP3, and enhanced survival of the cell under high light and cold stress.