Activity

The corticotropin-releasing factor receptor type 1 (CRF1R), a member of class B G-protein-coupled receptors (GPCRs), is a good drug target for treating depression, anxiety, and other stress-related neurodisorders. However, there is no approved drug targeting the CRF1R to date, partly due to inadequate structural information and its elusive activation mechanism. Here, by use of the crystal structures of its transmembrane domain (TMD) and the N-terminal extracellular domain (ECD) as a template, a full-length homology model of CRF1R was built and its complexes with peptide agonist urocortin 1 or small molecule antagonist CP-376395 were subjected to all-atom molecular dynamics simulations. We observed well preserved helical contents in the TMD through simulations, while the transmembrane (TM) helices showed clear rearrangements. The TM rearrangement is especially pronounced for the TM6 in the agonist-bound CRF1R system. The observed conformational changes are likely due to breakage of interhelical/inter-regional hydrogen bonds in the TMD. Telaglenastat Glutaminase inhibitor Dynamical network analysis identifies communities with high connections to TM6. Simulations reveal three key residues, Y3566.53, Q3847.49, and L3957.60, which corroborate experimental mutagenesis data, implying the important roles in the receptor activation. The observed large-scale conformational changes are related to CRF1R activation by agonist binding, providing guidance for ligand design.Manifested from the “tears of wine” to the “coffee-ring effect”, the directional transport of a liquid governed by the Marangoni effect is highly pervasive in our daily life and has brought a great number of applications. Similar to this surface tension gradient-dominated process, the fluid preferentially flows from the hot region to the cold region. In contrast to this perception, in this study, we report that water liquid deposited on a specially designed topological surface can flow from the low-temperature region to the high-temperature region in a spontaneous, long-range, and unidirectional manner. We show that such a behavior is mainly owing to a strong topological effect that outweighs the thermal gradient imposed along the surface. Moreover, the specific temperature range applied on the topological surface for the occurrence of such a unidirectional liquid transport phenomenon is also identified. Our findings would find important insights for developing next-generation cooling devices where a rapid flow from the condensation region to the evaporation/boiling region is preferred.Increasing working voltage of cathode has been identified as one of the most promising strategies to increase energy density of the lithium-ion batteries. It is of crucial importance to suppress side reactions and control the formation of a cathode electrolyte interface (CEI) on the cathode surface in a high voltage range. In this work, sulfur is utilized to increase the working voltage of LiNi0.5Co0.2Mn0.3O2(NCM 523) to 4.5 V as demonstrated by both the NCM523/Li half-cell and NCM 523/graphite full cell. When a tiny amount of sulfur (0.1 mg mL-1) is added to the blank electrolyte of ethylene carbonate (EC) and dimethyl carbonate (DMC) (37 by volume), the cycling stability and rate performance are greatly improved in the NCM523/Li half-cell. The capacity retention over 200 cycles at 170 mA g-1 (1.0 C) is increased from 61.2 to 82.0%. The capacity at a high current density of 850 mA g-1 (5.0 C) is increased from 92 mAh g-1 to 120 mAh g-1. Because the addition of sulfur also enhances the performance of the Li/graphite half-cell, improved performance is demonstrated by the NCM 523/graphite full cell as well. The mechanism is interpreted based on various characterizations. It is revealed that the preferential oxidation of sulfur at the cathode surface suppress decomposition of electrolyte solvent. Because only a tiny amount of sulfur is added into the electrolyte solution, excessive decomposition of sulfur is avoided, leading to improved electrochemical performance.Three-dimensional (3D) structures of V10O24·12H2O nanosheets coated with carbon (denoted as V10O24@C) are facially and cost-effectively fabricated by reducing the V2O5-based aqueous solution with ethanol under hydrothermal conditions. By using the 3D V10O24@C as the cathode of zinc-ion batteries, the as-obtained 3D V10O24@C sample delivers excellent charge-discharge cycling capability, superior rate performance, and reasonable specific capacity, and a specific capacity of ca. 133.3 mA h g-1 and a 94.1% capacity retention are achieved even after 10000 cycles at a high current density of 10 A g-1 (∼80 C). Furthermore, it provides a facile and scalable approach to synthesize the 3D structures of pure-phased vanadium oxide nanosheets or other nanoscale metal oxides coated with carbon.Anomalies in brain insulin signaling have been demonstrated to be involved in the pathology of Alzheimer disease (AD). In this context, the neuroprotective efficacy of an insulin sensitizer, rosiglitazone, has been confirmed in our previous study. In the present study, we hypothesize that a combination of an epigenetic modulator, vorinostat, along with rosiglitazone can impart improved gene expression of neurotrophic factors and attenuate biochemical and cellular alteration associated with AD mainly by loading these drugs in a surface modified nanocarrier system for enhanced bioavailability and enhanced therapeutic efficacy. Hence, in this study, rosiglitazone and vorinostat were loaded onto a poloxamer stabilized polymeric nanocarrier system and administered to mice in the intracerebroventricular streptozotocin (3 mg/kg) induced model of AD. Treatment with the free drug combination (rosiglitazone 5 mg/kg, vorinostat 25 mg/kg) for 3 weeks attenuated the behavioral, biochemical, and cellular alterations as compared to either treatment alone (rosiglitazone 10 mg/kg, vorinostat 50 mg/kg). Further, the coencapsulated nanoformulation (rosiglitazone 5 mg/kg, vorinostat 25 mg/kg) exerted better neuroprotective efficacy than the free drug combination as evidenced by improved behavioral outcome, reduced oxidative stress, and elevated levels of neurotrophic factors. In conclusion, the synergistic neuroprotective efficacy of rosiglitazone and vorinostat has been increased through the poloxamer stabilized polymeric nanocarrier system.